tcna1546pre Mouse Anti-Human IgM Antibody- prediluted

Western blot analysis of IgM antibody and Raji cell lysate.IHC analysis of FFPE human tonsil tissue and IgM antibody (MuHC2)
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Product Description
IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously.  IgM antibodies account for approximately 5%-10% of all the antibody in the body.

Information
ApplicationIHC-P
Species ReactivityHuman. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsHuman Mu heavy chain was used as the immunogen for this IgM antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesMuHC2
IsotypeMouse IgG1, kappa

Specifications
FormLiquid
Storage BufferPrediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Recommended DilutionPrediluted IHC only format : incubate for 30 min at RT (2)Due to differences in protocols and secondaries
the IgM antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling sections in 10mM citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information
Storage InstructionStore the IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP01871 & P20769
Gene ID3507 (human);
ReferencesProtein G
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