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Product Description
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
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Information
ApplicationFACS, IF, WB, IHC-P
Species ReactivityHuman. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsRecombinant heavy chain of human IgM (IM260 IM373 & ICO-30) was used as the immunogen for the Anti-IgM antibody cocktail.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesIM260 + IM373 + ICO-30
IsotypeMouse IgG1 and IgG2b, kappa
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Specifications
FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration1 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Anti-IgM antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.
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Misc Information
Storage InstructionStore the Anti-IgM antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein G affinity chromatography