Product Description
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Information
ApplicationFACS, IF, WB, IHC-P
Species ReactivityHuman. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsRecombinant heavy chain of human IgM (IM260 IM373 & ICO-30) was used as the immunogen for the Anti-IgM antibody cocktail.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesIM260 + IM373 + ICO-30
IsotypeMouse IgG1 and IgG2b, kappa
Specifications
FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration1 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Anti-IgM antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.
Misc Information
Storage InstructionStore the Anti-IgM antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein G affinity chromatography