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1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) drip mAb solution onto the tissue section and incubate at RT for 30 min.