tcna539pre Mouse Anti-Human IgM Antibody Cocktail- prediluted

Formalin-fixed, paraffin-embedded human tonsil stained with anti-IgM antibody (IM260 + IM373 + ICO-30)
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Product Description

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Information

ApplicationIHC-P
Species ReactivityHuman. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsRecombinant heavy chain of human IgM (IM260 IM373 & ICO-30) was used as the immunogen for the Anti-IgM antibody cocktail.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesIM260 + IM373 + ICO-30
IsotypeMouse IgG1 and IgG2b, kappa

Specifications

FormLiquid
Storage BufferPrediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Recommended DilutionPrediluted IHC only format : incubate for 30 min at RT (2)Optimal dilution of the Anti-IgM antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information

Storage InstructionStore the Anti-IgM antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein G affinity chromatography
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