Product Description
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Information
ApplicationFACS, IF, IHC-P
Species ReactivityHuman. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsHeavy chain of human IgM was used as the immunogen for the Anti-IgM antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesSPM188
IsotypeMouse IgG1, kappa
Specifications
FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration1 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Anti-IgM antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.
Misc Information
Storage InstructionStore the Anti-IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein G affinity chromatography