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Secondary Antibody
Recombinant Rabbit Anti-Human IgG4 Fc Antibody- sodium azide free
tcna4772saf
Recombinant Rabbit Anti-Human IgG4 Fc Antibody- sodium azide free
Sandwich ELISA with human plasma using recombinant Human IgG4 Fc antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human IgG4 using recombinant Human IgG4 Fc antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Recombinant Human IgG4 Fc antibody specifically reacts to hIgG4. No cross reactivity with IgG1, IgG2, IgG3.
ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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AVAILABLE SIZES
100ug
$
648.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
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Immunofluorescence (IF)
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Co-Immunoprecipitation (CoIP)
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Product Description
This recombinant Human IgG4 Fc antibody reacts to the Fc region of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
Information
Application
ELISA
Species Reactivity
Human
Host Species
Rabbit
Immunogen / Amino acids
Peptide corresponding to the Fc region of hIgG4 was used as the immunogen for this recombinant Human IgG4 Fc antibody.
Conjugation
Purified
Clonality
Recombinant Rabbit Monoclonal
Clones
RM217
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended Dilution
ELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection).The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 Fc antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Misc Information
Storage Instruction
Store the recombinant Human IgG4 Fc antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProt
P01861
Gene ID
3503
(human);
References
Purified
related data
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