tcna4771 Recombinant Rabbit Anti-Human IgG4 Antibody

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Product Description

This recombinant Human IgG4 antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.


ApplicationWB, ICC, IHC, FACS, ELISA
Species ReactivityHuman
Host SpeciesRabbit
Immunogen / Amino acidsPeptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Rabbit Monoclonal
IsotypeRabbit IgG


Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 1-10ug/ml
Immunohistochemistry: 1-10ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information

Storage InstructionStore the recombinant Human IgG4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Gene ID3503 (human);
ReferencesBiotin Conjugate
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