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Secondary Antibody
Recombinant Rabbit Anti-Human IgG3 Antibody
tcna4770
Recombinant Rabbit Anti-Human IgG3 Antibody
Sandwich ELISA with human IgG3 using recombinant Human IgG3 antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human plasma using recombinant Human IgG3 antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
IHC testing of FFPE human lymphoid tissue with recombinant Human IgG3 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows recombinant Human IgG3 antibody reacts only to hIgG3. No cross reactivity with IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of hIgG3. A serial dilution of recombinant Human IgG3 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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AVAILABLE SIZES
50ug
100ug
$
548.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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CELLS
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Product Description
This recombinant Human IgG3 antibody reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Information
Application
ICC, IHC, FACS, ELISA
Species Reactivity
Human
Host Species
Rabbit
Immunogen / Amino acids
Human IgG3 was used as the immunogen for this recombinant Human IgG3 antibody.
Conjugation
Biotin Conjugate
Clonality
Recombinant Rabbit Monoclonal
Clones
RM119
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended Dilution
ELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Human IgG3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Misc Information
Storage Instruction
Store the recombinant Human IgG3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProt
P01860
Gene ID
3502
(human);
References
Biotin Conjugate
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