tcna4755saf Recombinant Goat Anti-Mouse IgG Fab Antibody- sodium azide free

ELISA of mouse immunoglobulins shows the recombinant Mouse IgG Fab antibody reacts to the Fab region of IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human/rat/rabbit IgG.ELISA Titration: the plate was coated with different amounts of mouse IgG. A serial dilution of recombinant Mouse IgG Fab antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
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Product Description
This recombinant Mouse IgG Fab antibody reacts to the Fab region of mIgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE or human/rat/rabbit IgG..

Information
ApplicationIP, ICC, IHC, FACS, ELISA
Species ReactivityMouse
Host SpeciesGoat
Immunogen / Amino acidsMouse IgG was used as the immunogen for this recombinant Mouse IgG Fab antibody.
ConjugationPurified
ClonalityRecombinant Goat Monoclonal
ClonesRMG05
IsotypeGoat IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended DilutionELISA: 0.05ug/ml - 1ug/ml
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry (FFPE): 0.5-2ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG Fab antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Mouse IgG Fab antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Gene IDN/A (human);
ReferencesPurified
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