tcna4752saf Recombinant Rabbit Anti-Mouse IgG2c Antibody- sodium azide free

Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of the recombinant Mouse IgG2c antibody. This mAb reacts to nonreduced IgG2c.ELISA of IgG from BalB/C and C57BL6 shows the recombinant Mouse IgG2c antibody reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a.ELISA Titration: the plate was coated with different amounts of mouse IgG2c. A serial dilution of the recombinant Mouse IgG2c antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Product Description
This recombinant Mouse IgG2c antibody reacts to the Fc region of mIgG2c. No cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

Information
ApplicationWB (non-reduced only), IP, ICC, IHC, FACS, ELISA
Species ReactivityMouse
Host SpeciesRabbit
Immunogen / Amino acidsMouse IgG2c was used as the immunogen for this recombinant Mouse IgG2c antibody.
ConjugationPurified
ClonalityRecombinant Rabbit Monoclonal
ClonesRM223
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended DilutionELISA: 0.01ug/ml - 0.2ug/ml
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry (FFPE): 0.5-2ug/ml (1)
Western Blot (non-reduced only): 0.5-2ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2c antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Mouse IgG2c antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtN / A
Gene ID404711 (human);
ReferencesPurified
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