tcna1486 Rabbit Anti-Human IgG Heavy Chain Antibody

IHC staining of FFPE human tonsil tissue with recombinant IgG antibody (clone GHC07-2R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
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$526.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
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Product Description

This antibody reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. It is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Information

ApplicationIHC-P
Species ReactivityHuman. Other species not tested.
Host SpeciesRabbit
Immunogen / Amino acidsHuman Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody.
ConjugationUnconjugated
ClonalityRecombinant Rabbit Monoclonal
ClonesGHC07-2R
IsotypeRabbit IgG, kappa

Specifications

FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration0.2 mg/ml
Recommended DilutionImmunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)The stated application concentrations are suggested starting points. Titration of the recombinant IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information

Storage InstructionStore the recombinant IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP01857
ReferencesProtein A affinity chromatography
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