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Secondary Antibody
Recombinant Rabbit Anti-Human IgG Heavy Chain Antibody
tcna1276
Recombinant Rabbit Anti-Human IgG Heavy Chain Antibody
IHC testing of FFPE human tonsil with recombinant IgG antibody (clone IG1707R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant IgG antibody (clone IG1707R) as confirmation of integrity and purity.
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AVAILABLE SIZES
20ug
100ug
$
526.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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CELLS
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Product Description
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Information
Application
FACS, IF, IHC-P
Species Reactivity
Human. Other species not known.
Host Species
Rabbit
Immunogen / Amino acids
Human Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody.
Conjugation
Unconjugated
Clonality
Recombinant Rabbit Monoclonal
Clones
IG1707R
Isotype
Rabbit IgG, kappa
Specifications
Form
Liquid
Storage Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration
0.2 mg/ml
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
The optimal dilution of the recombinant IgG antibody for each application should be determined by the researcher.
Misc Information
Storage Instruction
Store the recombinant IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProt
P01857
References
Protein A affinity chromatography
related data
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