tcna1267 Mouse Anti-Human IgG4 Antibody

IHC testing of human tonsil with IgG4 antibody (clone IGHG4/1345). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.
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Data sheet

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Product Description

The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells. IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.

Information

ApplicationFACS, IHC-P, IF, WB
Species ReactivityHuman
Host SpeciesMouse
Immunogen / Amino acidsA human recombinant protein corresponding to the Fc region was used as the immunogen for this IgG4 antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesIGHG4/1345
IsotypeMouse IgG1

Specifications

FormLiquid
Storage Buffer0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Concentration0.2 mg/ml
Recommended DilutionFACS: 0.5-1ug/10^6 cells
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC (FFPE): 0.5-1ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols
secondaries and substrates may require the IgG4 antibody to be titered up or down for optimal performance.

Misc Information

Storage InstructionStore the IgG4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP01861
Gene ID3503 (human);
ReferencesProtein G affinity chromatography
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