tcna1003 Mouse Anti-Human IgG Antibody Cocktail

Formalin-fixed, paraffin-embedded human tonsil stained with IgG antibody (IG217 + IG266)
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Product Description
This mAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of gamma heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.

Information
ApplicationFACS, IF, IHC-P
Species ReactivityHuman. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsPurified human IgG was used as the immunogen for this IgG antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesIG217 + IG266
IsotypeMouse IgG1 + IgG2a

Specifications
FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended DilutionFlow Cytometry (1ug/million cells in 0.1ml)
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the IgG antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information
Storage InstructionStore the IgG antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP01857 / P01859 / P01860 / P01861
ReferencesProtein G affinity chromatography
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