tcna1000saf Mouse Anti-Human IgG Antibody- sodium azide free

Formalin-fixed, paraffin-embedded human tonsil stained with IgG antibody (IG217)SDS-PAGE Analysis of Purified, BSA-Free IgG Antibody (clone IG217). Confirmation of Integrity and Purity of the Antibody.
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$498.00 ORDERING INFORMATION International
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Product Description

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Information

ApplicationFACS, IF, IHC-P
Species ReactivityHuman. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsPurified human Ig Gamma Chain was used as the immunogen for this IgG antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesIG217
IsotypeMouse IgG1, kappa

Specifications

FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (Formalin/paraffin) 0.5-1ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the IgG antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information

Storage InstructionStore the IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein G affinity chromatography
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