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Primary Antibody
Mouse anti DDDDK-Tag Monoclonal Antibody
tcba13665
Mouse anti DDDDK-Tag Monoclonal Antibody
Western blot analysis of over-expressed DDDDK-tagged protein in 293T cell using DDDDK-tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb. Blue: DAPI for nuclear staining.
Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody. A mock served as negative control and over-expressed 293T cell lysate served as positive control.
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AVAILABLE SIZES
50ul
100ul
200ul
$
113.60
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.
Information
Application
WB, IHC, IF, IP
Host Species
Mouse
Immunogen / Amino acids
A synthetic Peptide of human DDDDK-Tag
Conjugation
Unconjugated
Clonality
Monoclonal
Isotype
IgG
Specifications
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:2000 - 1:5000
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:100
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Purification
Affinity purification
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