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Primary Antibody
ACTB Polyclonal Antibody
tcba13641
ACTB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using β-actin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using ACTB antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Formaldehyde
Methanol
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Heat mediated
Enzymatic
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Product Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Information
Application
WB, IHC, IF
Research Area
Epigenetics & Nuclear Signaling, Epigenetic Modifications, Epigenetic Modifications_Methylation, Epigenetics & Nuclear Signaling, Epigenetic Modifications, Epigenetic Modifications_Methylation,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human ACTB (NP_001092.1).
Conjugation
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Specifications
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
BRWS1;PS1TP5BP1
SwissProt
P60709
Gene ID
60
(human);
Calculated Molecular Weight
41kDa
Purification
Affinity purification
Cellular Location
Cytoplasm,cytoskeleton,
related data
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