tcba13639 ACTB Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using β-actin antibody at 1:80000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.Western blot analysis of extracts of NIH/3T3 cells, using β-actin antibody at 1:10000-1:2560000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.Immunohistochemistry of paraffin-embedded rat liver using β-actin antibody at dilution of 1:500 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using β-actin antibody at dilution of 1:500 (40x lens).Immunohistochemistry of paraffin-embedded rat spleen using β-actin antibody at dilution of 1:500 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using β-actin antibody at dilution of 1:500 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using β-actin antibody at dilution of 1:500 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody at dilution of 1:500 (40x lens).Immunohistochemistry of paraffin-embedded mouse spleen using β-actin antibody at dilution of 1:500 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using β-actin antibody at dilution of 1:500 (40x lens).
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Product Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Information
ApplicationWB, IHC, IF
Research AreaEpigenetics & Nuclear Signaling, Epigenetic Modifications, Epigenetic Modifications_Methylation, Epigenetics & Nuclear Signaling, Epigenetic Modifications, Epigenetic Modifications_Methylation,
Species ReactivityHuman, Mouse, Rat
Host SpeciesMouse
Immunogen / Amino acidsFusion protein of human β-actin
ConjugationUnconjugated
ClonalityMonoclonal
IsotypeIgG

Specifications
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:10000 - 1:100000
IHC 1:100- 1:200
IF 1:50- 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
SwissProtP60709
Gene ID60 (human);
Calculated Molecular Weight41kDa
PurificationAffinity purification
Cellular LocationCytoplasm,cytoskeleton,
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