tcba3402 TEAD1 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of MCF7 cells using TEAD1 antibody.Chromatin immunoprecipitation analysis extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Product Description
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

Information
ApplicationWB, IHC, IF, ChIP
Research AreaCancer, Hippo pathway, Cardiovascular, Developmental Biology, Epigenetics,
Species ReactivityHuman, Mouse
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
CHIP 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesAA;NTEF-1;REF1;TCF-13;TCF13;TEAD-1;TEF-1
SwissProtP28347
Gene ID7003 (human);
Calculated Molecular Weight40kDa/47kDa
PurificationAffinity purification
Cellular LocationNucleus,
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