tcba2510 RPN1 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using RPN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded human colon using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody at dilution of 1:100 (20x lens).Immunohistochemistry of paraffin-embedded mouse spinal cord using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody at dilution of 1:100 (40x lens).
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Product Description
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Information
ApplicationWB, IHC
Research AreaRNA Binding Protein(RBP),
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesOST1;RBPH1
SwissProtP04843
Gene ID6184 (human);
Calculated Molecular Weight68kDa
PurificationAffinity purification
Cellular LocationEndoplasmic reticulum membrane,Melanosome,Single-pass type I membrane protein,
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