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Primary Antibody
Phospho-RB1-S807 mAb
tcba13517
Phospho-RB1-S807 mAb
Western blot analysis of extracts of A549 cells, using Phospho-RB1-S807 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-RB1-S807 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using Phospho-RB1-S807 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Phospho-RB1-S807 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-RB1-S807 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human spleen using Phospho-RB1-S807 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse colon using Phospho-RB1-S807 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using Phospho-RB1-S807 antibody.
Immunofluorescence analysis of CRC cells using Phospho-RB1-S807 antibody.
Immunofluorescence analysis of A549 cells using Phospho-RB1-S807 antibody.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Information
Application
WB, IHC, IF
Research Area
Cancer, Cell cycle, Cell Biology, Cell Cycle, Epigenetics,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
OSRC;p105-Rb;pp110;PPP1R130;pRb;RB
SwissProt
P06400
Gene ID
5925
(human);
Calculated Molecular Weight
106kDa
Purification
Affinity purification
Cellular Location
Nucleus,
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