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Primary Antibody
PLK4 Polyclonal Antibody
tcba12830
PLK4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PLK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody at dilution of 1:100 (40x lens).
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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Loading Type
UG
CELLS
Treatment
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Exposure
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Formaldehyde
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Heat mediated
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Product Description
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Information
Application
WB, IHC
Research Area
Cancer, Cell cycle, Cell Biology,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
MCCRP2;SAK;STK18
SwissProt
O00444
Gene ID
10733
(human);
Calculated Molecular Weight
104kDa/105kDa/108kDa
Purification
Affinity purification
Cellular Location
Cleavage furrow,Cytoplasm,Nucleus,centriole,centrosome,cytoskeleton,microtubule organizing center,nucleolus,
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