tcba11428 SUZ12 Polyclonal Antibody

KOVALIDATED
Western blot analysis of extracts of various cell lines, using SUZ12 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using SUZ12 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using SUZ12 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody at dilution of 1:100 (40x lens).Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody. Western blot was performed from the immunoprecipitate using SUZ12 antibody at a dilition of 1:500.
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Product Description
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

Information
ApplicationWB, IHC, IP
Research AreaChromatin Remodeling,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCHET9;JJAZ1
SwissProtQ15022
Gene ID23512 (human);
Calculated Molecular Weight83kDa
PurificationAffinity purification
Cellular LocationNucleus,
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