tcba10885 MAP1LC3B Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using MAP1LC3B antibody at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.Western blot analysis of extracts of pig liver, using MAP1LC3B antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded rat heart using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using MAP1LC3B antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
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Product Description
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Information
ApplicationWB, IHC, IF
Research AreaCancer, Autophagy pathway, Neuroscience, Cardiovascular, Metabolism,
Species ReactivityHuman, Mouse, Rat, Pig
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a
SwissProtQ9GZQ8
Gene ID81631 (human);
Calculated Molecular Weight14kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Cytoplasmic vesicle,Endomembrane system,Lipid-anchor,autophagosome,autophagosome membrane,cytoskeleton,
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