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Primary Antibody
SPN Polyclonal Antibody
tcba10148
SPN Polyclonal Antibody
Western blot analysis of extracts of THP-1 cells, using SPN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SPN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using SPN antibody at dilution of 1:100 (40x lens).
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Exposure
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Formaldehyde
Methanol
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Heat mediated
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Product Description
The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response.
Information
Application
WB, IHC
Research Area
Cancer, Stem cells, Apoptosis, Immunology,
Species Reactivity
Human, Mouse
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
CD43;GALGP;GPL115;LSN
SwissProt
P16150
Gene ID
6693
(human);
Calculated Molecular Weight
40kDa
Purification
Affinity purification
Cellular Location
Membrane,Single-pass type I membrane protein,
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