tcba10146 SNRPA Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using SNRPA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using SNRPA Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using SNRPA Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using SNRPA antibody.
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Product Description
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.

Information
ApplicationWB, IHC, IF
Research Area,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesMud1;U1-A;U1A
SwissProtP09012
Gene ID6626 (human);
Calculated Molecular Weight31kDa
PurificationAffinity purification
Cellular LocationNucleus,
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