tcba10073 BCAP29 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using BCAP29 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.Immunohistochemistry of paraffin-embedded rat kidney using BCAP29 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using BCAP29 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using BCAP29 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human prostate using BCAP29 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using BCAP29 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse spleen using BCAP29 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using BCAP29 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using BCAP29 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using BCAP29 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC-12 cells using BCAP29 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.

Information
ApplicationWB, IHC, IF
Research AreaCancer, Apoptosis, AKT pathway, Immunology, PI3K-Akt pathway,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
SwissProtQ9UHQ4
Gene ID55973 (human);
Calculated Molecular Weight28kDa/40kDa
PurificationAffinity purification
Cellular LocationEndoplasmic reticulum membrane,Multi-pass membrane protein,
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