tcba9653 ATP5B Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using ATP5B Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.Immunohistochemistry of paraffin-embedded human liver injury using ATP5B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using ATP5B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using ATP5B antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC-12 cells using ATP5B antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Product Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Information

ApplicationWB, IHC, IF
Research AreaCancer, Neuroscience, Metabolism, Neurodegeneration,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesATPMB;ATPSB;HEL-S-271
SwissProtP06576
Gene ID506 (human);
Calculated Molecular Weight56kDa
PurificationAffinity purification
Cellular LocationMitochondrion,Mitochondrion inner membrane,
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