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Primary Antibody
DOK1 Polyclonal Antibody
tcba9574
DOK1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DOK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DOK1 antibody. Blue: DAPI for nuclear staining.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Heat mediated
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Product Description
The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene.
Information
Application
WB, IHC, IF
Research Area
Cancer, Cardiovascular, Metabolism,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
P62DOK;pp62
SwissProt
Q99704
Gene ID
1796
(human);
Calculated Molecular Weight
19kDa/37kDa/52kDa
Purification
Affinity purification
Cellular Location
Cytoplasm,Nucleus,perinuclear region,
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