tcba9562 RBPJ Polyclonal Antibody

Western blot analysis of extracts of mouse lung, using RBPJ antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded human stomach using RBPJ Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using RBPJ Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using RBPJ Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using RBPJ Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using RBPJ Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using RBPJ Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using RBPJ antibody. Blue: DAPI for nuclear staining.
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Product Description
The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.

Information
ApplicationWB, IHC, IF
Research AreaStem cells, Developmental Biology, Notch pathway, Epigenetics,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesAOS3;CBF1;csl;IGKJRB;IGKJRB1;KBF2;RBP-J;RBPJK;RBPSUH;SUH
SwissProtQ06330
Gene ID3516 (human);
Calculated Molecular Weight45kDa/47kDa/51kDa/54kDa/55kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,
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