tcba9449 PRDM14 Polyclonal Antibody

Western blot analysis of extracts of rat teatis, using PRDM14 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded rat liver using PRDM14 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using PRDM14 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human colon carcinoma using PRDM14 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human placenta using PRDM14 antibody at dilution of 1:200 (40x lens).Immunofluorescence analysis of HeLa cells using PRDM14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using PRDM14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Product Description
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.

Information
ApplicationWB, IHC, IF, IP, ChIP
Research Area,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:100
ChIP 1:20 - 1:100

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesPFM11
SwissProtQ9GZV8
Gene ID63978 (human);
Calculated Molecular Weight64kDa
PurificationAffinity purification
Cellular LocationNucleus,
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