tcba9404 PRPF3 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using PRPF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using PRPF3 antibody. Blue: DAPI for nuclear staining.
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Product Description
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.

Information
ApplicationWB, IHC, IF, IP
Research AreaNeuroscience,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesHPRP3;HPRP3P;PRP3;Prp3p;RP18;SNRNP90
SwissProtO43395
Gene ID9129 (human);
Calculated Molecular Weight31kDa/77kDa
PurificationAffinity purification
Cellular LocationNucleus speckle,
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