tcba7776 USO1 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using USO1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry of paraffin-embedded human kidney using USO1 antibody at dilution of 1:200 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using USO1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver cancer using USO1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using USO1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using USO1 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using USO1 antibody. Blue: DAPI for nuclear staining.
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Product Description
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.

Information
ApplicationWB, IHC, IF
Research AreaRNA Binding Protein(RBP),
Species ReactivityHuman, Mouse
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesP115;TAP;VDP
SwissProtO60763
Gene ID8615 (human);
Calculated Molecular Weight107kDa/109kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Golgi apparatus membrane,Peripheral membrane protein,cytosol,
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