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Primary Antibody
TRAP1 Polyclonal Antibody
tcba7737
TRAP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TRAP1 antibody. Blue: DAPI for nuclear staining.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Exposure
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Acetone
Formaldehyde
Methanol
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Heat mediated
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Product Description
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
Information
Application
WB, IHC, IF
Research Area
Cancer, Apoptosis, Cell Biology, Metabolism, RNA Binding Protein(RBP),
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
HSP75;HSP75;HSP90L;TRAP-1
SwissProt
Q12931
Gene ID
10131
(human);
Calculated Molecular Weight
74kDa/80kDa
Purification
Affinity purification
Cellular Location
Mitochondrion,Mitochondrion inner membrane,Mitochondrion matrix,
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