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Primary Antibody
EEF1G Polyclonal Antibody
tcba7712
EEF1G Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EEF1G antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse brain using EEF1G antibody (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EEF1G antibody (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EEF1G antibody (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EEF1G antibody (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EEF1G antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EEF1G antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A431 cells using EEF1G antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Exposure
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Heat mediated
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Product Description
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
Information
Application
WB, IHC, IF
Research Area
,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
EF1G;GIG35
SwissProt
P26641
Gene ID
1937
(human);
Calculated Molecular Weight
50kDa/56kDa
Purification
Affinity purification
related data
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