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Primary Antibody
CREB1 Polyclonal Antibody
tcba7477
CREB1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CREB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using CREB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CREB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using CREB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using CREB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CREB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CREB1 antibody at dilution of 1:100 (40x lens).
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Antigen Retrieval
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Heat mediated
Enzymatic
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Product Description
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Information
Application
WB, IHC
Research Area
Cancer, MAPK pathway, Neuroscience, Immunology, Innate Immunity, Neurodegeneration, MAPK/p38 pathway, MAPK/ERK pathway, Epigenetics,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
CREB;CREB-1
SwissProt
P16220
Gene ID
1385
(human);
Calculated Molecular Weight
25kDa/35kDa/36kDa
Purification
Affinity purification
Cellular Location
Nucleus,
related data
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