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Primary Antibody
EDF1 Polyclonal Antibody
tcba7404
EDF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EDF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EDF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using EDF1 antibody at dilution of 1:100 (40x lens).
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants.
Information
Application
WB, IHC
Research Area
Cancer, Cardiovascular, Cell Biology, Metabolism, Lipid Metabolism pathway, RNA Binding Protein(RBP), Epigenetics,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
CFAP280;EDF-1;MBF1
SwissProt
O60869
Gene ID
8721
(human);
Calculated Molecular Weight
15kDa/16kDa
Purification
Affinity purification
Cellular Location
Cytoplasm,Nucleus,
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