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Primary Antibody
TOLLIP Polyclonal Antibody
tcba7350
TOLLIP Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TOLLIP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human stomach using TOLLIP antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TOLLIP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using TOLLIP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using TOLLIP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TOLLIP antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TOLLIP antibody. Blue: DAPI for nuclear staining.
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$
140.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Formaldehyde
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Product Description
This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.
Information
Application
WB, IHC, IF
Research Area
Immunology, Innate Immunity, Toll-like Receptor pathway,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
IL-1RAcPIP
SwissProt
Q9H0E2
Gene ID
54472
(human);
Calculated Molecular Weight
22kDa/30kDa
Purification
Affinity purification
Cellular Location
Cytoplasm,
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