tcba7304 Caspase-3 Rabbit Polyclonal antibody [KO Validated]

KOVALIDATED
Western blot analysis of extracts of Jurkat cells, using CASP3 antibody at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.Western blot analysis of extracts from normal (control) and Caspase-3 knockout (KO) 293T cells, using Caspase-3 antibody (A2156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat spleen using CASP3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human placenta using CASP3 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of C6 cells using CASP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using CASP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using CASP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunohistochemistry of paraffin-embedded rat spleen using Caspase-3 antibody at dilution of 1:100 (40x lens).Customer ReviewCustomer Review
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Product Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Information

ApplicationWB, IHC, IF
Research AreaCancer, Apoptosis, Neuroscience, Cell Biology, Metabolism, Neurodegeneration,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3
SwissProtP42574
Gene ID836 (human);
Calculated Molecular Weight31kDa
Observed Molecular Weight17kDa, 35kDa
PurificationAffinity purification
Positive ControlJurkat
Cellular LocationCytoplasm,
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