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Primary Antibody
IKBKB Polyclonal Antibody
tcba7235
IKBKB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IKBKB antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lung cancer using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IKBKB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IKBKB antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using IKBKB antibody. Blue: DAPI for nuclear staining.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Product Description
The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.
Information
Application
WB, IHC, IF
Research Area
Cancer, Apoptosis, Cardiovascular, Cell Biology, Immunology, Innate Immunity, NF-κB pathway, Toll-like Receptor pathway,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
IKK-beta;IKK2;IKKB;IMD15;NFKBIKB
SwissProt
O14920
Gene ID
3551
(human);
Calculated Molecular Weight
29kDa/79kDa/85kDa/86kDa
Purification
Affinity purification
Cellular Location
Cytoplasm,Membrane raft,Nucleus,
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