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Primary Antibody
RAB27A Polyclonal Antibody
tcba7088
RAB27A Polyclonal Antibody
KO
VALIDATED
Western blot analysis of extracts of U-937 cells, using RAB27A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.
Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using RAB27A Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using RAB27A Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using RAB27A antibody. Blue: DAPI for nuclear staining.
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
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Exposure
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Formaldehyde
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Heat mediated
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Product Description
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Information
Application
WB, IHC, IF
Research Area
Cancer, Apoptosis,
Species Reactivity
Human, Mouse
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
GS2;HsT18676;RAB27;RAM
SwissProt
P51159
Gene ID
5873
(human);
Calculated Molecular Weight
24kDa
Purification
Affinity purification
Cellular Location
Late endosome,Lipid-anchor,Lysosome,Melanosome,Membrane,
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