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Primary Antibody
MAP1LC3B Polyclonal Antibody
tcba5708
MAP1LC3B Polyclonal Antibody
Western blot analysis of extracts of mouse brain, using MAP1LC3B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 2min.
Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using MAP1LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MAP1LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using MAP1LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using MAP1LC3B antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using MAP1LC3B antibody at dilution of 1:100. C6 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
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International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Information
Application
WB, IHC, IF
Research Area
Cancer, Autophagy pathway, Neuroscience, Cardiovascular, Metabolism,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a
SwissProt
Q9GZQ8
Gene ID
81631
(human);
Calculated Molecular Weight
14kDa
Purification
Affinity purification
Cellular Location
Cytoplasm,Cytoplasmic vesicle,Endomembrane system,Lipid-anchor,autophagosome,autophagosome membrane,cytoskeleton,
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