tcba4725 S100A10 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using S100A10 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.Immunohistochemistry of paraffin-embedded rat ovary using S100A10 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human colon using S100A10 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using S100A10 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human mammary cancer using S100A10 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse leydig cells using S100A10 antibody at dilution of 1:100 (40x lens).
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Product Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.

Information
ApplicationWB, IHC
Research AreaCardiovascular, Calcium pathway,
Species ReactivityHuman, Mouse, Rat, Monkey
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative Names42C;ANX2L;ANX2LG;CAL1L;Ca[1];CLP11;GP11;p10;P11
SwissProtP60903
Gene ID6281 (human);
Calculated Molecular Weight11kDa
PurificationAffinity purification
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