Product Description
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Information
ApplicationWB, IHC, IF, IP
Research AreaCancer, Apoptosis, AKT pathway, Neuroscience, Cell Biology, Metabolism, Neurodegeneration, PI3K-Akt pathway,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
IsotypeIgG
Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesBCL2L4; BAX
Calculated Molecular Weight4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed Molecular Weight20kDa
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
PurificationAffinity purification
Positive Control293T, HeLa, Raji, RAW264.7, Mouse lung, Rat lung
Cellular LocationCytoplasm,Cytoplasm,Mitochondrion membrane,Single-pass membrane protein,