tcba1664 c-Jun Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using JUN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.Immunohistochemistry of paraffin-embedded human colon using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human lung using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human placenta using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse testis using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using c-Jun antibody (tcba1663) at dilution of 1:100 (40x lens).Immunofluorescence analysis of C6 cells using c-Jun antibody (tcba1663) at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using c-Jun antibody (tcba1663) at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS cells using c-Jun antibody (tcba1663) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Product Description
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Information
ApplicationWB
Research AreaCancer, Immunology, Innate Immunity, Epigenetics,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesAP-1;AP1;c-Jun
SwissProtP05412
Gene ID3725 (human);
Calculated Molecular Weight35kDa
PurificationAffinity purification
Cellular LocationNucleus,
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