Product Description
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Information
ApplicationWB, IHC, IF
Research AreaCancer, Cell cycle, Autophagy pathway, AKT pathway, mTOR pathway, Cardiovascular, Cell Biology, Metabolism, DNA Damage/Repair, TGF-β SMAD pathway, JAK STAT pathway, AMPK pathway, PI3K-Akt pathway, Epigenetics,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsRecombinant fusion protein containing a sequence corresponding to amino acids 2388-2487 of human mTOR (NP_004949.1).
IsotypeIgG
Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Calculated Molecular Weight288kDa
Observed Molecular Weight289kDa
SequenceEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGL
PurificationAffinity purification
Positive ControlU-87MG, HeLa, K-562, Mouse liver
Cellular LocationCytoplasm,Cytoplasmic side,Endoplasmic reticulum membrane,Golgi apparatus membrane,Lysosome,Mitochondrion outer membrane,Nucleus,PML body,Peripheral membrane protein,