tcba1650 EGFR Polyclonal Antibody

KOVALIDATED
Western blot analysis of extracts of various cell lines, using EGFR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.Immunohistochemistry of paraffin-embedded rat lung using EGFR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using EGFR antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of HeLa cells using EGFR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC12 cells using EGFR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Order Now

AVAILABLE SIZES

$140.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Information

ApplicationWB, IHC, IF, IP
Research AreaCancer, Tumor biomarkers,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications

FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100

Misc Information

Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesERBB;ERBB1;HER1;mENA;NISBD2;PIG61
SwissProtP00533
Gene ID1956 (human);
Calculated Molecular Weight44kDa/69kDa/77kDa/134kDa
PurificationAffinity purification
Cellular LocationCell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Golgi apparatus membrane,Nucleus membrane,Nucleus,Secreted,Single-pass type I membrane protein,
Get valuable resources and offers directly to your email.