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Primary Antibody
PHB Monoclonal Antibody
tcba1516
PHB Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using PHB antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse stomach using PHB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using PHB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using PHB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PHB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PHB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PHB antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using PHB antibody.
Immunofluorescence analysis of HepG2 cells using PHB antibody.
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$
140.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Product Description
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Information
Application
WB, IHC, IF, IP
Research Area
Cancer, Cell cycle, Apoptosis, Cell Biology, Metabolism,
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Isotype
IgG
Specifications
Form
Liquid
Storage Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilution
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Misc Information
Storage Instruction
Store at -20℃. Avoid freeze / thaw cycles.
Alternative Names
HEL-215;HEL-S-54e;PHB1
SwissProt
P35232
Gene ID
5245
(human);
Calculated Molecular Weight
17kDa/29kDa
Purification
Affinity purification
Cellular Location
Mitochondrion inner membrane,
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