tcba1513 CTGF Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using CTGF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.Immunohistochemistry of paraffin-embedded rat heart using CTGF antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast using CTGF antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using CTGF antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of C6 cells using CTGF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using CTGF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS cells using CTGF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS cells using CTGF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using CTGF antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Product Description
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.

Information
ApplicationWB, IHC, IF
Research AreaCardiovascular, Immunology,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:200 - 1:500
IHC 1:50 - 1:200
IF 1:20 - 1:50

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesCCN2;HCS24;IGFBP8;NOV2
SwissProtP29279
Gene ID1490 (human);
Calculated Molecular Weight35kDa/38kDa
PurificationAffinity purification
Cellular LocationSecreted,extracellular matrix,extracellular space,
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