tcba912 SFN Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using SFN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Immunohistochemistry of paraffin-embedded rat lung using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver injury using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human kidney using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human esophagus using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat lung using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat liver using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat spleen using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat kidney using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human liver using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using SFN Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using SFN Antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of MCF-7 cells using SFN antibody. Blue: DAPI for nuclear staining.
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.

Information
ApplicationWB, IHC, IF
Research AreaCancer, Stem cells, Cell cycle, Wnt pathway, MAPK pathway, Neuroscience, Developmental Biology, Cell Biology, Cell Cycle, MAPK/ERK pathway,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesYWHAS
SwissProtP31947
Gene ID2810 (human);
Calculated Molecular Weight24kDa/27kDa
PurificationAffinity purification
Cellular LocationCytoplasm,Nucleus,Secreted,
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