tcba586 PARP1 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human mammary cancer using PARP1 antibody at dilution of 1:100 (40x lens).Immunofluorescence analysis of U2OS cells using PARP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Product Description
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Information
ApplicationWB, IHC, IF
Research AreaCancer, DNA Damage/Repair, NF-κB pathway, RNA Binding Protein(RBP), Epigenetics,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:1000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
Alternative NamesADPRT;ADPRT1;ADPRT1;ARTD1;pADPRT-1;PARP;PARP-1;PPOL
SwissProtP09874
Gene ID142 (human);
Calculated Molecular Weight113kDa
PurificationAffinity purification
Cellular LocationNucleus,nucleolus,
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